Jess™ Automated Western Blotting Services
Western blotting is a powerful and widely utilized laboratory technique for identifying and quantifying proteins based on their molecular weight and expression levels. This method is invaluable in drug development and manufacturing, providing insights into protein expression, post-translational modifications, and protein-protein interactions. Beyond traditional Western blotting services, Avance Biosciences offers ProteinSimple’s Jess™, an advanced system that performs capillary-based immunoassays and fully automated Western blot analysis, significantly enhancing precision, efficiency, and throughput in protein characterization.
Our Expertise
We have integrated the Jess™ Platform into our suite of analytical tools to enhance our capacity for supporting drug product manufacturing, preclinical GLP, and clinical GLP. Leveraging our years of experience, we provide support in Western blotting assay development, validation, and sample testing to address our clients’ specific needs.
Selected Applications:
Application
Description
Protein Expression Analysis
The Jess™ system accurately quantifies the expression levels of biologic drugs, ensuring that the correct therapeutic protein is produced in sufficient quantities.
Potency Assay
Jess™ facilitates protein expression potency assays designed for upstream and downstream process development. It provides a fit-for-purpose, efficient, simple, and cost-effective method for quantifying protein expression, enabling relative potency assays within a multi-attribute platform approach.
Protein Identity and Purity Assay
Jess™ employs a multi-attribute method to assess both identity and purity in a single assay. For example, antibodies specific to VP1/VP2/VP3 capsid proteins are used for identity measurements, while total protein detection is used for purity assessments.
Empty/Full Content Ratio
Jess™ offers automated measurements of empty/full AAV capsid content ratios with high sensitivity, reproducibility, and scalability. This multi-attribute platform method effectively characterizes multiple AAV serotypes in complex samples.
Viral Titer and VP Capsid Protein Ratio Measurements
Jess™ accurately quantifies VP1, VP2, and VP3 protein concentrations and their molar ratios, providing a comprehensive tool for optimizing and maintaining the quality of AAV gene therapy products.
Process-Related Impurities
Jess™ immunoassays offer highly sensitive detection of residual proteins from therapeutic protein and vaccine development processes. These include host cell protein (HCP) contamination, Protein A, GFP, and bovine serum albumin (BSA).
Vector Analysis
The Jess™ automated multi-attribute platform provides scalable at-line analytics for lentiviral vectors, including measurements of p24 titer, identity, purity, stability, capsid content ratio, and transduction efficiency.
Targeted Protein Degradation Assay
Jess™ supports the execution of quantitative DC50 curves with a high-throughput protein degradation western blot workflow, delivering reproducible and fully quantitative data for targeted protein degradation studies.