Phage Testing for Non-E. coli Cell Banks
The use of a wider range of microbial cells beyond E. coli in biopharma is expanding the potential for novel therapeutics and bioprocesses. It’s crucial to develop detection methods for lytic and lysogenic phages in these cells to ensure product safety and process stability, as phage contamination can disrupt production and compromise product integrity.
Our Expertise
Equipped with more than 20 years of experience of testing lytic and lysogenic phage in E coli cell banks, Avance Biosciences team is also skilled in developing and validating novel assays to detect lytic and lysogenic phages in other microbial organisms, such as Salmonella, Staphylococcus, and Streptococcus.
Phage Testing Services
Service
Description
Assay Development
- Identify positive and negative control strains.
- Identify permissive host strain suitable for phage testing.
- Develop a method for lysogenic phage induction
- Develop a plaque testing protocol for lytic phage
Assay Optimization
- Optimize PFUs for quantifiable plaques.
- Standardize bacterial lawn preparation.
- Set incubation conditions for clear plaque visibility
- Experiment with different induction conditions for specific strains
Validation
- Develop validation plans for lytic and lysogenic phage detection, respective
- Validate assay specificity
- Validate assay limit of detection
- Prepare a custom SOP(s) for sample testing
Sample Testing
- Test samples with validated method
- TAT usually <2 weeks