Flow Cytometry Services
Flow cytometry is instrumental in drug development and manufacturing processes, providing invaluable insights into cellular behavior and function that are crucial for ensuring the efficacy and safety of pharmaceutical products. This powerful technology enables the identification and characterization of diverse cellular populations within heterogeneous samples, encompassing genetically modified cells, immune cells, stem cells, and cancer cells. From assessing product quality in manufacturing to assessing treatment efficacy and safety in preclinical and clinical studies, flow cytometry serves as an indispensable tool to support every phase of the drug development process.
Our Expertise
Avance Biosciences scientific team boasts over several decades of combined experience in flow cytometry assays. With a proven track record in custom assay development, validation, and sample testing, our team excels in providing creative solutions to address our clients’ specific needs, ensuring efficient, sensitive, and reproducible assays. Leveraging the advanced CytoFLEX multicolor flow cytometer and years of our flow cytometry experience, we’re well positioned to support assays in drug product manufacturing, preclinical GLP, and clinical GLP.
Flow Cytometry Services
Application
Description
Immunophenotyping
Flow cytometry is widely used to identify and characterize various immune cell subsets based on the expression of surface and intracellular markers. This application is crucial in immunology research, vaccine development, and monitoring immune responses.
Cancer Research
Flow cytometry helps in the detection and characterization of cancer cells. It is used to analyze cell surface markers, DNA content, and apoptosis, providing insights into cancer progression, prognosis, and treatment efficacy.
Cell Cycle Analysis
Flow cytometry is employed to analyze the cell cycle by measuring DNA content in cells. This application is essential in understanding cell proliferation, growth, and the effects of drugs on cell cycle progression.
Apoptosis Detection
Flow cytometry can detect and quantify apoptotic cells by measuring specific markers such as annexin V and propidium iodide. This application is important in studying programmed cell death and the effects of cytotoxic drugs.
Biomarker Discovery
Flow cytometry is used to discover and validate biomarkers for various diseases. By analyzing the expression of proteins and other molecules, researchers can identify potential biomarkers for diagnosis, prognosis, and therapeutic targets.
Minimal Residual Disease (MRD) Detection
In hematological malignancies, flow cytometry is used to detect minimal residual disease (MRD) with high sensitivity. This application helps in assessing treatment efficacy and predicting relapse.
Microbial and Environmental Analysis
Flow cytometry is employed to analyze microorganisms in various environmental samples. It is used to study microbial populations, monitor water quality, and detect pathogens.
Stem Cell Research
Flow cytometry is crucial in stem cell research for characterizing stem cells and their differentiation status. It also aids in sorting specific stem cell populations for further study or therapeutic use.
Pharmacodynamics (PD) Studies
In drug development, flow cytometry is used to monitor the effects of drugs on specific cell populations and pathways. This application helps in understanding the mechanism of action and optimizing therapeutic regimens.
Clinical Diagnostics
Flow cytometry is used in clinical diagnostics to diagnose and monitor various diseases, including hematological disorders, immunodeficiencies, and infectious diseases. It provides rapid and accurate analysis of patient samples.
Functional Assays
Flow cytometry-based functional assays can assess various cell functions, including cytokine production, phagocytosis, and cell signaling. These assays are important in both basic research and clinical applications.