Lentivirus Product Support Services

Lentiviruses are a type of retrovirus, a family of RNA viruses that can integrate their genetic material into the host cell’s DNA. They are characterized by their ability to infect both dividing and non-dividing cells, making them a versatile tool in various fields of biology and medicine.

About

Lentiviruses have gained significant attention due to their use in gene therapy and molecular biology research. They are commonly employed for gene delivery into target cells, allowing the introduction or modification of specific genes. Lentiviral vectors, which are modified forms of the virus, have become valuable tools for genetic manipulation, including gene knockdown or overexpression, as well as gene editing techniques like CRISPR/Cas9.

To meet the demands of this rapidly growing field, Avance Biosciences’ Lentivirus Quality Control Testing Services offer comprehensive solutions to deliver cutting-edge results with confidence.

Key Lentivirus Product Support Services

Critical Quality Attributes

Assay

Description

Identity

Sequence Confirmation

Verify the sequence of Lentivirus genome by Sanger Sequencing or NGS

Long Read NGS

Sequence Lentivirus genome by long read Nanopore sequencing

Purity

Residual Plasmid DNA

Quantify residual plasmid DNA using qPCR or ddPCR.

Capsid content (empty/full ratio)

Measure the percent of full to empty capsids by CE-SDS

Capsid purity Analysis

Assess the purity of Lentivirus capsid proteins using CE-SDS or Jess

Purity

Residual Plasmid DNA

Quantify residual plasmid DNA using qPCR or ddPCR.

Residual host cell DNA

Quantify residual host cell DNA using qPCR or ddPCR.

Residual Host Cell Protein

Determine presence and quantitate residual host cell proteins by ELISA

Potency

Vector Genome Titer

Quantify the number of vector genomes using qPCR or ddPCR.

Cell-based assay

Develop cell-based assay for evaluating post-transduction endpoint (mRNA expression, protein production)

Genetic Identity

Vector Genome Integrity

Confirm the integrity of the vector genome using QPCR/ddPCR or Bioanalyzer or CE

Safety

Bioburden

USP <61>, <62>

Endotoxin

USP <85>

Safety

Bioburden

USP <61>, <62>

Sterility

USP <71>

Other

Appearance

USP <790>

USP <791>

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